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Experiment 5

Experiments

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  • Apparatus
  • Diagram
  • Method
  • Calculation
  • Graph
  • Conclusion
  • Precautions
  • Sources of Error





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  • To investigate the effect of substrate concentration on the rate of enzyme activity

    1. Perform experiment 1 at different substrate concentrations.
  • Test tube A: 5 starch
  • Test tube B: 4 starch + 1 distilled water
  • Test tube C: 3 starch + 2 distilled water
  • Test tube D: 2 starch + 3 distilled water.
    A control must be set up for each of the substrate concentrations.

    2. Keep pH constant by using buffer pH 8.

    3. Keep enzyme concentration constant by using the same volume of the same amylase solution each time.

    4. Keep temperature constant by using a water bath set at 37.

    5. Every minute remove a sample from each test tube and test with Benedict solution.

    6. Record the time taken for maltose to be produced i.e. to get a brick red colour.

    7. Rate = 1/Time. The longer the time the slower the rate.

    8. Graph the results with substrate concentration on the x-axis.













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