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Experiment 4

Experiments

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  • Apparatus
  • Diagram
  • Method
  • Calculation
  • Graph
  • Conclusion
  • Precautions
  • Sources of Error





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  • To investigate the effect of enzyme concentration on the rate of enzyme activity

    1. Perform experiment 1 at different enzyme concentrations.
  • Test tube A: 5 amylase
  • Test tube B: 4 amylase + 1 distilled water
  • Test tube C: 3 amylase + 2 distilled water
  • Test tube D: 2 amylase + 3 distilled water.
    A control must be set up for each of the enzyme concentrations.

    2. Keep pH constant by using buffer pH 8.

    3. Keep substrate concentration constant by using the same volume of the same starch solution each time.

    4. Keep temperature constant by using a water bath set at 37.

    5. Every minute remove a sample from each test tube and test with Iodine.

    6. Record the time taken for the disappearance of the starch i.e. the time taken to get a yellow-brown.

    7. Rate = 1/Time. The longer the time the slower the rate.

    8. Graph the results with enzyme concentration on the x-axis.













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