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Experiment 3

Experiments

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  • Apparatus
  • Diagram
  • Method
  • Calculation
  • Graph
  • Conclusion
  • Precautions
  • Sources of Error





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  • To investigate the effect of temperature on the rate of enzyme activity

    1. Perform experiment 1 at different temperatures.
    Use an ice bath for 0, use water baths set at different temperatures e.g. 20, 37, 80. A control must be set up for each of the different temperatures.

    2. Keep pH constant by using buffer pH 8.

    3. Keep substrate concentration constant by using the same volume of the same starch solution each time.

    4. Keep enzyme concentration constant by using the same volume of the same amylase solution each time.

    5. Every minute remove a sample from each test tube and test with Iodine.

    6. Record the time taken for the disappearance of the starch i.e. the time taken to get a yellow-brown.

    7. Rate = 1/Time. The longer the time the slower the rate.

    8. Graph the results with temperature on the x - axis.













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