Experiments
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To investigate the effect of amylase on starch
1. Heat amylase solution; starch solution, and distilled water to 37
in a water bath.2. Mix 5
of the starch solution and 5 of the amylase solution in a test tube.(test tube A)3. Mix 5
of the starch solution and 5 of the distilled water in a test tube (test tube B - control).4. Place the test tubes in a water-bath set at 37
.5. Add a few drops of buffer pH 8 to keep the pH constant.
6. Every minute remove a sample from each test tube and test with Iodine.
Result
Test tube A: Turns yellow-brown - the starch has been broken down.
Test tube B: Always blue-black, starch present.
7. Every minute remove a sample from each test tube and test with Benedict's solution.
Result
Test tube A: Turns brick red - the starch has been broken down into maltose.
Test tube B: Remains blue - no maltose present.
